基于Label-free技术的鹿角与鹿骨蛋白组分比较

doi:10.11843/j.issn.0366-6964.2017.12.008

Abstract

Abstract:

The study aimed to carry out the comparison proteomic analysis of wapiti antler and bone based on Label-free. In this paper, the wapiti antlers and bone aged 130 d were selected as experimental material, then the protein components of antler and bone were analyzed by using the Label-free proteomics methods and bioinformatics methods. The results showed that a total of 1 138 proteins were identified, including 934 deer bone proteins and 835 antler proteins. Through the functional annotation of proteins successfully identified, deer bone proteins were specifically enriched in the process of low-density lipoprotein receptor particle metabolism, substantia nigra development, angiogenesis and regulation of cell apoptotic signaling pathway. The antler proteins were enriched in the peptide hormone processing, regulation of activated T cell proliferation and other biological processes. The proteins related to peptide hormone processing process were CPE, CPN1, ECE1, and the proteins related to regulation of activated T cell proliferation were IGF2, PRKAR1A, PYCARD. A total of 335 differentially expressed proteins (fold change>2, P<0.05) were screened out by SPSS software. Among them, 75 proteins were up-regulated in deer bone and 260 proteins were up-regulated in antlers. In addition, cathelicidin 1 and cathelicidin 5 were highly expressed in antlers, while cathelicidin 6 was highly expressed in the deer bone.The results fill the blank in the study of antler and deer bone protein components, and lay the foundation for the further study on the pharmacological active substances of antler and deer bones.

CLC Number:

S825.2

ZHANG Ran-ran, LIU Hua-miao, WANG Lei, ZHOU Yong-na, TANG Fu-quan, XIA Shu-zhong, XING Xiu-mei. Comparison Proteomic Analysis of Wapiti Antler and Bone Based on Label-free[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2286-2292.

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Comparison Proteomic Analysis of Wapiti Antler and Bone Based on Label-free

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